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KMID : 0903520060490040334
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2006 Volume.49 No. 4 p.334 ~ p.338
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Isolation and Quantitative Analysis of Inosine as an Index Component from Larva of Allomirina dichotoma
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Lee Jin-Hee
Song Myoung-Chong
Yang Hye-Jung
Bang Myun-Ho
Chung Dong-Jun
Kim Young-Chai
Kim Se-Young
Rho Yeong-Deok
Chung Sung-Hyun
Baek Nam-In
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Abstract
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A repeated silica gel column chromatography of n-BuOH fraction from the larva of Allomirina dichotoma afforded a purified nucleic acid, which was identified as inosine on the basis of the spectroscopic data including IR, MS and NMR. The HPLC analysis of the index component using silica based NH2-column exhibited the contents in the hot air-dried and Far-IR-dried larvae of Allomirina dichotoma as 0.003 % and 0.055 %, respectively.
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KEYWORD
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Allomirina dichotoma, larva, inosine, index component, HPLC, quantitative analysis
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